FISH Test Menu

The Cytogenetics Laboratory maintains an extensive and continuously expanding fluorescence in situ hybridization (FISH) test menu for hematologic malignancies, prenatal testing, and other clinical applications.

Representative probes and panels are listed below. Additional probes and customized testing strategies are available based on clinical indication.

Plasma Cell Neoplasms / Multiple Myeloma

MM Panel

  • 1p36/1q21 (CHD5, S100A10)
  • 13q14 (D13S319, LAMP1)
  • 11;14 (CCND1, IGH)
  • IGH (14q32) (IGHx2)
  • TP53 (17p13) (TP53, CEP17)
  • 5,9,15 (D5S23/D5S721, CEP9, CEP15)

MM Translocation Panel

  • 4;14 (FGFR3, IGH)
  • 6;14 (CCND3, IGH)
  • 8;14 (CEP8, MYC, IGH)
  • 14;16 (IGH, MAF)
  • 14;20 (IGH, MAFB)

 

Myeloid Neoplasms

MDS Panel

  • 5q31 (D5S23/D5S721, EGR1)
  • 7q31 (CEP7, D7S486)
  • 8,20 (CEP8, D20S108)

MPN Panel

  • 9;22 (ABL1, BCR)
  • 1p36/1q21 (CHD5, S100A10)
  • 8,20 (CEP8, D20S108)
  • 13q (D13S319, LAMP1)

Eosinophilic Neoplasm Panel

  • 9;22 (ABL1, BCR)
  • PDGFRA (4q12) (SCFD2, LNX, 3’PDGFRA/KIT)
  • PDGFRB (5q33) (PDGFRBx2)
  • FGFR1 (8p11) (FGFR1, CEP8)
  • JAK2 (9p24) (JAK2x2)

AML Panel

  • 8;21 (RUNX1T1, RUNX1)
  • CBFB (16q22) (CBFBx2)
  • KMT2A (MLL) (KMT2Ax2)
  • MECOM (3q26) (MECOMx2)
  • ETV6 (12p13) (ETV6x2)
  • 17p13.1 (TP53, CEP17)
  • NUP98 (11p15) (NUP98x2)
  • 6;9 (DEK, NUP214)

Monocytic AML Panel

  • KMT2A (MLL) (KMT2Ax2)
  • CBFB (16q22) (CBFBx2)
  • NUP98 (11p15) (NUP98x2)

 

Lymphoid Neoplasms

CLL Panel

  • MYB (6q23) (MYBx2)
  • 11,17 (ATM, TP53)
  • 12,13 (CEP12, D13S319, LAMP1)
  • 13,21 (RB1, D21S341/D21S342)
  • 11;14 (CCND1, IGH)
  • IGH (14q32) (IGHx2)

LGL Panel

  • 14;18 (IGH, BCL2)
  • IGH (14q32) (IGHx2)
  • BCL2 (18q21) (BCL2x2)
  • BCL6 (3q27) (BCL6x2)

HGL Panel

  • 14;18 (IGH, BCL2)
  • 8;14 (CEP8, MYC, IGH)
  • IGH (14q32) (IGHx2)
  • BCL2 (18q21) (BCL2x2)
  • BCL6 (3q27) (BCL6x2)
  • MYC (8q24) (MYCx2)

T-Cell Receptor Rearrangement Panel

  • TRA/D (14q11) (TRA/Dx2)
  • TRB (7q34) (TRBx2)
  • TCL1 (14q32) (TCL1x2)

B-ALL Panel

  • 9;22 (ABL1, BCR)
  • KMT2A (MLL) (KMT2Ax2)
  • 12;21 (ETV6, RUNX1)
  • 1;19 (PBX1, TCF3)
  • 3,7,9,17 (CEP3, CEP7, CDKN2A, CEP17)
  • 4,10,17 (CEP4, CEP10, CEP17)

B-ALL Reflex Panel

  • CDKN2A (9p21) (CDKN2A, CEP9)
  • Xp22.33/Yp11.23 (CRLF2x2)
  • 8;14 (CEP8, MYC, IGH)
  • IGH (14q32) (IGHx2)
  • MYC (8q24) (MYCx2)

Ph-Like B-ALL Panel

  • PDGFRB (5q33) (PDGFRBx2)
  • JAK2 (9p24) (JAK2x2)

 

Prenatal and Reproductive Applications

Prenatal Panel: Female

  • XY,18 (CEPXx2, CEPYx0, CEP18x2)
  • 13q14 (D13S319, LAMP1)
  • 12;21 (ETV6, RUNX1)

Prenatal Panel: Male

  • XY,18 (CEPXx1, CEPYx1, CEP18x2)
  • 13q14 (D13S319, LAMP1)
  • 12;21 (ETV6, RUNX1)

Products of Conception (POC) Panel

  • XY,18 (CEPXx1, CEPYx1, CEP18x2)
  • 13q14 (D13S319, LAMP1)
  • 12;21 (ETV6, RUNX1)
  • 16,22 (CEP16, BCR)

 

Additional Testing

Additional probes and customized testing strategies are available based on specimen type, clinical indication, and diagnostic context.
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